Evaluation of sera with a low signal to cut-off ratio using two chemiluminescent assays for detecting Hepatitis C Virus, and their correlation with the detection of Viral RNA. / Valoración de sueros con bajo índice S/CO utilizando 2 sistemas de quimioluminiscencia para la detección de anticuerpos frente al virus de la hepatitis C y su correlación con la detección de ARN viral.

OBJECTIVE: All commercial assays used to measure the presence of Hepatitis C virus (HCV) antibodies set cut-off points to categorise the results, but the problem of false positive results in screening hepatitis C sera is well known. The aim of this study was to evaluate the results obtained by two chemiluminescent assays in selected sera, and compare these results with the detection of viral RNA in the specimens studied. MATERIAL AND METHODS: Two hundred reactive sera (positive) were selected, although with a low signal to cut-off ratio (S/CO), were selected, using two chemiluminescent assays and were then subjected to genome amplification. RESULTS AND DISCUSSION: Viral RNA could be only be detected in 8 (4%) of the selected specimens. Taking these results into account, we believe that the design of the current chemiluminescent assays do not provide sufficient specificity when they are used as the only tests for the diagnosis of hepatitis C.

Qualidade física de um latossolo vermelho sob sistema de semeadura direta e cultivo convencional / Physical quality of an oxisol under no-tillage and conventional tillage

Para garantir a sustentabilidade dos sistemas agrícolas, a compreensão e a quantificação do impacto do uso e manejo do solo na sua qualidade física são de fundamental importância. Nesse sentido, objetivou-se com este trabalho avaliar a qualidade de um Latossolo Vermelho sob cultivo convencional (CC) e sistema de semeadura direta (PD), por meio de diferentes indicadores físicos. Os sistemas de uso e manejo do solo foram: CC e PD por sete e oito anos consecutivos (solo de textura média) e CC e PD por nove e dez anos consecutivos (solo de textura argilosa). Foram determinadas, nas camadas de 0-0,10; 0,10-0,20 e 0,20-0,30 m, a resistência do solo à penetração, a porosidade total, a macroporosidade e a microporosidade, a retenção de água no solo, o índice S, a densidade, a densidade do solo máxima e também a densidade do solo relativa. Foi verificada grande variação da resistência do solo à penetração ao longo do ciclo das culturas da soja e do milho, sendo que seus maiores valores foram constatados nas camadas superficiais. Dentre os sistemas de manejo, o PD apresentou maior resistência do solo à penetração. Dentre os sistemas de manejo, o CC apresentou melhores resultados em relação aos indicadores de qualidade física do solo.

The understanding and quantification of the impact of tillage systems in their physical quality are fundamental in the development of sustainable agricultural systems. This study aimed to evaluate the quality of an Oxisol under conventional tillage (CT) and no-tillage system (NT), through different physical indicators. The management systems were: CT and NT for seven or eight consecutive years (medium textured soil) and CT and NT by nine and ten consecutive years (clay soil). Were determined, at the layers 0-0.10, 0.10-0.20; 0.20-0.30 m, soil resistance to penetration, total soil porosity, macroporosity and microporosity, soil water retention, S index, soil bulk density, maximum density and relative bulk density. Was observed great variation of soil resistance to penetration throughout the soybean and corn cycles, with its highest values were found in the surface layers. The NT showed greater resistance to penetration. Among the management systems, the results against indicators of soil physical quality were similar.